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从枯草芽孢杆菌营养细胞和孢子中分离出的DNA-蛋白质复合物模式的差异。

Differences in pattern of a DNA protein complex isolated from vegetative cells and spores of Bacillus subtilis.

作者信息

Gruissem W

出版信息

Mol Gen Genet. 1978 Feb 16;159(2):213-8. doi: 10.1007/BF00270896.

DOI:10.1007/BF00270896
PMID:416335
Abstract

A DNA protein complex has been isolated from vegetative cells and spores of Bacillus subtilis. Properties of the DNA protein complex prepared from vegetative cells were studied and SDS gel electrophoresis was employed to compare the different DNase-untreated and -treated DNA protein complexes. It is concluded that proteins are associated with the DNA and differences in protein pattern in polyacrylamide gels indicates the involvement of DNA-binding proteins in the regulation of spore formation.

摘要

已从枯草芽孢杆菌的营养细胞和孢子中分离出一种DNA-蛋白质复合物。对从营养细胞制备的DNA-蛋白质复合物的性质进行了研究,并采用SDS凝胶电泳来比较不同的未用DNase处理和用DNase处理的DNA-蛋白质复合物。得出的结论是,蛋白质与DNA相关联,聚丙烯酰胺凝胶中蛋白质模式的差异表明DNA结合蛋白参与了孢子形成的调控。

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本文引用的文献

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8
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