Soriano F, Cherry J D
Appl Microbiol. 1967 Mar;15(2):243-4. doi: 10.1128/am.15.2.243-244.1967.
A virus plaque method that consistently gives good visual contrast for macroscopic observation and also permits microscopic study is described. Cells are grown in plastic flasks and the gelled overlay medium can be of any desired agar concentration or volume. A fixing solution is used prior to removal of the agar overlay, and dye is added to the fixing solution or staining can follow fixation and agar removal. The bottom of the flask with the fixed monolayer is separated from the rest of the container and handled as a slide. A new mounting medium is described.
本文描述了一种病毒蚀斑方法，该方法能始终为宏观观察提供良好的视觉对比度，同时也允许进行微观研究。细胞在塑料培养瓶中生长，凝胶覆盖培养基可以是任何所需的琼脂浓度或体积。在去除琼脂覆盖层之前使用固定液，可将染料添加到固定液中，或者在固定和去除琼脂之后进行染色。带有固定单层细胞的培养瓶底部与容器的其余部分分离，并作为载玻片处理。本文还描述了一种新的封固介质。
