Fogel A, Plotkin S A
J Virol. 1969 Feb;3(2):157-63. doi: 10.1128/JVI.3.2.157-163.1969.
When tested on RK(13) cell cultures, strains of rubella virus could be differentiated by their ability to form small or large plaques. Large plaques were produced by the HPV-77 and Cendehill strains, and also by a laboratory stock strain (West Point), after only 14 passages in RK(13) culture. Five wild-type rubella viruses, isolated and passaged only a few times in African green monkey kidney tissue culture, grew well in RK(13) cell culture, but they were sensitive to agar inhibitors and, therefore, formed small plaques. On the other hand, RA27/3, an attenuated strain grown in WI-38 human fibroblast cells, developed low titers in RK(13) cells and also produced small plaques. We concluded that the morphological differences between small-plaque and large-plaque viruses depended on their sensitivity to agar inhibitors and on the pH of the medium during plaque formation.
在RK(13)细胞培养物上进行测试时，风疹病毒株可根据其形成小斑块或大斑块的能力进行区分。HPV - 77株和森德希尔株以及实验室储备株（西点株）在RK(13)培养物中仅传代14次后就能产生大斑块。5株野生型风疹病毒在非洲绿猴肾组织培养物中仅分离和传代几次，在RK(13)细胞培养物中生长良好，但它们对琼脂抑制剂敏感，因此形成小斑块。另一方面，在WI - 38人成纤维细胞中培养的减毒株RA27/3在RK(13)细胞中的滴度较低，也产生小斑块。我们得出结论，小斑块病毒和大斑块病毒之间的形态差异取决于它们对琼脂抑制剂的敏感性以及斑块形成过程中培养基的pH值。
