Rice C E, Smith H J, Cochrane D
Can J Comp Med Vet Sci. 1967 Feb;31(2):37-42.
Electrophoretic examinations using cellulose acetate strips were made on 17 serum samples collected at two-week intervals from each of three calves before and for about four months after they were placed on parasite-infected pastures in June, 1964. Four additional serum samples taken during the winter months when the animals were stabled and two obtained after they were again exposed to infected pastures in June 1965, were also examined. Two control animals of the same age, that were placed on clean pasture in 1964 and not exposed to infected pasture until June 1965, were bled on the same dates and their sera examined in parallel. All five calves showed an increase in the proportion and amount of gamma-globulin in their serum as they increased in age but the gain was greatest in the two exposed calves that developed the highest complement-fixing titres with nematode antigens. Complement-fixing titres rose slowly in the other exposed calf and its serum gamma-globulin level remained relatively comparable to that of the two controls.
1964年6月，从三头小牛身上每隔两周采集一次血清样本，共采集了17份，在它们被放置在感染寄生虫的牧场之前以及之后大约四个月的时间里进行了醋酸纤维素条电泳检查。另外还检查了在冬季动物圈养期间采集的四份血清样本，以及1965年6月它们再次暴露于感染牧场后获得的两份血清样本。选取了两头同龄对照动物，它们于1964年被放置在干净的牧场，直到1965年6月才暴露于感染牧场，在相同日期采血并对其血清进行平行检测。随着年龄增长，所有五头小牛血清中γ-球蛋白的比例和含量都有所增加，但在两头暴露的小牛中增加幅度最大，它们对线虫抗原产生了最高的补体结合滴度。另一头暴露的小牛补体结合滴度上升缓慢，其血清γ-球蛋白水平与两头对照动物的血清γ-球蛋白水平相对相当。
