Hargis G K, Reynolds W A, Williams G A, Kawahara W, Jackson B, Bowser E N, Pitkin R M
Clin Chem. 1978 Apr;24(4):595-601.
We describe a radioimmunoassay for rhesus monkey and human immunoreactive calcitonin, in which a selected goat anti-human calcitonin antiserum, 131I-labeled synthetic human calcitonin tracer, and purified synthetic human calcitonin standards were used. The mean basal concentration of calcitonin in normal monkey plasma (254 microgram/liter) was not significantly different from that in normal human plasma (217 microgram/liter). The data indicate that the method is sensitive (lower limit of detection, 5 ng/liter), specific, accurate, and reproducible (coefficient of variation, 1-11% over a wide range of concentrations). Monkey calcitonin response to changes in plasma calcium concentration is similar to that in man, with significant correlation between calcium and calcitonin concentrations. Use of this radioimmunoassay for monkey and human calcitonin allows the monkey to be used in further studies of factors regulating secretion, function, and metabolism of calcitonin under various experimental conditions that would not be feasible in man.
我们描述了一种用于恒河猴和人免疫反应性降钙素的放射免疫测定法，该方法使用了一种选定的山羊抗人降钙素抗血清、¹³¹I标记的合成人降钙素示踪剂和纯化的合成人降钙素标准品。正常猴血浆中降钙素的平均基础浓度（254微克/升）与正常人血浆中的平均基础浓度（217微克/升）无显著差异。数据表明该方法灵敏（检测下限为5纳克/升）、特异、准确且可重复（在很宽的浓度范围内变异系数为1% - 11%）。猴降钙素对血浆钙浓度变化的反应与人相似，钙浓度与降钙素浓度之间存在显著相关性。使用这种用于猴和人降钙素的放射免疫测定法，使得在各种对人不可行的实验条件下，能够利用猴进一步研究调节降钙素分泌、功能和代谢的因素。
