Roos B A, Deftos L J, Roberts G, Wilson P, Bundy L
J Lab Clin Med. 1976 Aug;88(2):173-82.
We have developed a sensitive immunoassay for murine calcitonin which can measure the basal concentration of the hormone in peripheral plasma of rats as well as the calcitonin, extracted from rat thyroid tissue. Human calcitonin was used for tracer, standard, and antibody production. Assay methods were devised to minimize artifacts which have been shown to spuriously influence immunoassay performance. The basal plasma concentration of calcitonin in rats was 6 to 75 pg. per milliliter. Infusions of calcium, pentagastrin, and PGE2 produced a 2- to 7-fold increase in plasma calcitonin. Elevated concentrations of calcitonin were demonstrated in tumor specimens obtained from the second and fourth generations of a transplanted rat medullary thyroid carcinoma. Our results demonstrate that calcitonin circulates in the peripheral plasma of rats in the basal state and that hormone concentrations change during functional tests of secretion. This assay will permit studies of calcitonin secretion in physiological and pathophysiological states in the rat.
我们已经开发出一种用于检测小鼠降钙素的灵敏免疫测定法，该方法能够测量大鼠外周血浆中该激素的基础浓度，以及从大鼠甲状腺组织中提取的降钙素。使用人降钙素作为示踪剂、标准品和用于抗体生产。设计了检测方法以尽量减少已证明会虚假影响免疫测定性能的假象。大鼠血浆中降钙素的基础浓度为每毫升6至75皮克。输注钙、五肽胃泌素和前列腺素E2可使血浆降钙素增加2至7倍。在从第二代和第四代移植性大鼠甲状腺髓样癌获得的肿瘤标本中，降钙素浓度升高。我们的结果表明，降钙素在基础状态下在大鼠外周血浆中循环，并且在分泌功能测试期间激素浓度会发生变化。该测定法将有助于研究大鼠在生理和病理生理状态下的降钙素分泌情况。
