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Recalibration of the Pseudomonas aeruginosa strain PAO chromosome map in time units using high-frequency-of-recombination donors.利用高频重组供体对铜绿假单胞菌PAO菌株染色体图谱进行时间单位的重新校准。
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VARIATIONS IN RESTRICTION AND MODIFICATION OF BACTERIOPHAGE FOLLOWING INCREASE OF GROWTH TEMPERATURE OF PSEUDOMONAS AERUGINOSA.铜绿假单胞菌生长温度升高后噬菌体限制与修饰的变化
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Genetic circularity of the Pseudomonas aeruginosa PAO chromosome.铜绿假单胞菌PAO染色体的遗传环状结构
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A map order for his I, one of the genetic regions controlling histidine biosynthesis in Pseudomonas aeruginosa, using the transducing phage F116.利用转导噬菌体F116对铜绿假单胞菌中控制组氨酸生物合成的基因区域之一his I进行图谱排序。
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通过种间交配构建的铜绿假单胞菌R'质粒的分离与鉴定

Isolation and characterization of Pseudomonas aeruginosa R' plasmids constructed by interspecific mating.

作者信息

Morgan A F

出版信息

J Bacteriol. 1982 Feb;149(2):654-61. doi: 10.1128/jb.149.2.654-661.1982.

DOI:10.1128/jb.149.2.654-661.1982
PMID:6799492
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC216555/
Abstract

Plasmid R68.45 was used to construct R' plasmids carrying a maximum of 4 to 5 map minutes of the Pseudomonas aeruginosa PAO chromosome by interspecific mating, using P. putida PPN as the recipient. These R' plasmids were used to determine the map location of the amiE locus and to identify tentatively a number of P. putida auxotrophic mutations. Some of these R' plasmids could not be maintained in recombination-deficient P. aeruginosa strains.

摘要

质粒R68.45被用于通过种间交配构建携带最多4至5个基因图谱分钟的铜绿假单胞菌PAO染色体的R'质粒,以恶臭假单胞菌PPN作为受体。这些R'质粒被用于确定amiE基因座的图谱位置,并初步鉴定一些恶臭假单胞菌营养缺陷型突变。其中一些R'质粒无法在重组缺陷型铜绿假单胞菌菌株中维持。