通过种间交配构建的铜绿假单胞菌R'质粒的分离与鉴定
Isolation and characterization of Pseudomonas aeruginosa R' plasmids constructed by interspecific mating.
作者信息
Morgan A F
出版信息
J Bacteriol. 1982 Feb;149(2):654-61. doi: 10.1128/jb.149.2.654-661.1982.
Abstract
Plasmid R68.45 was used to construct R' plasmids carrying a maximum of 4 to 5 map minutes of the Pseudomonas aeruginosa PAO chromosome by interspecific mating, using P. putida PPN as the recipient. These R' plasmids were used to determine the map location of the amiE locus and to identify tentatively a number of P. putida auxotrophic mutations. Some of these R' plasmids could not be maintained in recombination-deficient P. aeruginosa strains.
摘要
质粒R68.45被用于通过种间交配构建携带最多4至5个基因图谱分钟的铜绿假单胞菌PAO染色体的R'质粒，以恶臭假单胞菌PPN作为受体。这些R'质粒被用于确定amiE基因座的图谱位置，并初步鉴定一些恶臭假单胞菌营养缺陷型突变。其中一些R'质粒无法在重组缺陷型铜绿假单胞菌菌株中维持。
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6
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7
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10
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本文引用的文献
1
VARIATIONS IN RESTRICTION AND MODIFICATION OF BACTERIOPHAGE FOLLOWING INCREASE OF GROWTH TEMPERATURE OF PSEUDOMONAS AERUGINOSA.
Virology. 1965 Apr;25:634-42. doi: 10.1016/0042-6822(65)90091-7.
2
Linkage map of Escherichia coli K-12, edition 6.
Microbiol Rev. 1980 Mar;44(1):1-56. doi: 10.1128/mr.44.1.1-56.1980.
3
Isolation and characterization of an R-prime plasmid from Rhizobium meliloti.
J Bacteriol. 1980 Jan;141(1):121-8. doi: 10.1128/jb.141.1.121-128.1980.
4
Genetic circularity of the Pseudomonas aeruginosa PAO chromosome.
J Bacteriol. 1981 Jan;145(1):145-55. doi: 10.1128/jb.145.1.145-155.1981.
5
Direct repetition of a 1.2 Md DNA sequence is involved in site-specific recombination by the P1 plasmid R68.
Gene. 1980 Sep;10(4):319-28. doi: 10.1016/0378-1119(80)90152-3.
6
Transduction and the clustering of genes in fluorescent Pseudomonads.
Proc Natl Acad Sci U S A. 1968 May;60(1):168-75. doi: 10.1073/pnas.60.1.168.
7
8
Genetics of Pseudomonas.
Bacteriol Rev. 1969 Sep;33(3):419-43. doi: 10.1128/br.33.3.419-443.1969.
9
Control of pyrimidine biosynthesis in Pseudomonas aeruginosa.
J Bacteriol. 1968 Nov;96(5):1732-41. doi: 10.1128/jb.96.5.1732-1741.1968.
10
The regulation of tryptophan biosynthesis in Pseudomonas aeruginosa.
Mol Gen Genet. 1973 Mar 1;121(2):117-32. doi: 10.1007/BF00277526.
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