Hurst R E, Nakamura N, West S S
Prep Biochem. 1978;8(1):37-56. doi: 10.1080/00327487808068217.
New methods for isolation and fractionation by partition are described and compared with existing techniques. Substantially purer products were isolated by partition as compared to precipitation with hexadecylpyridinium chloride. The glycosaminoglycans isolated fron Furth murine mastocytoma tumor were found to consist of 78-80% heparin, 12-13% chondroitin sulfate, and 8-9% hyaluronate. Dermatan sulfate was not detected. Two heparin-like glycosaminoglycans could be isolated by partition fractionation in the phase system 1-butanol/aqueous NaCl containing hexadecylpyridinium chloride. The composition of one was typical of heparins. However, the other glycosaminoglycan contained only 0.47 moles N-sulfate/mole uronate, but had electrophoretic and partition properties characteristic of heparin.
本文描述了通过分配进行分离和分级分离的新方法，并与现有技术进行了比较。与用十六烷基氯化吡啶沉淀相比，通过分配分离得到的产品纯度更高。从弗思小鼠肥大细胞瘤中分离出的糖胺聚糖由78 - 80%的肝素、12 - 13%的硫酸软骨素和8 - 9%的透明质酸盐组成。未检测到硫酸皮肤素。在含有十六烷基氯化吡啶的1-丁醇/氯化钠水溶液相体系中，通过分配分级分离可以分离出两种类肝素糖胺聚糖。其中一种的组成是典型的肝素。然而，另一种糖胺聚糖每摩尔糖醛酸仅含0.47摩尔N-硫酸盐，但具有肝素特有的电泳和分配特性。
