Nader H B, Marx W, Spolter L
Biochim Biophys Acta. 1980 Sep 1;631(3):463-78. doi: 10.1016/0304-4165(80)90022-7.
The glycosaminoglycan fractions of four murine mastocytomas (AB-CBF1-MCT-1, AB-CXBG-MCT-1, AB-CXBI-MCT-1, and Furth), were isolated and characterized using microelectrophoresis, degradation with specific mucopolysaccharidases, paper chromatography and, in some instances, ion-exchange chromatography. Intramuscular and ascites tumors and tumors cultured in vitro were used. The glycosaminoglycans were labeled with [35S]sulfate and , in a few instances, with D-[6-3H]glucosamine. The glycosaminoglycan fraction obtained from the AB-CBF1-MCT-1 mastocytoma cultured in vitro consisted predominantly of dermatan sulfate-like material. On the other hand, the major components of the glycosaminoglycan fractions of the AB-CXBG-MCT-1 and AB-CXBI-MCT-1 and Furth tumors were observed to behave like heparin or heparan sulfate. In most of the mastocytoma samples examined, chondroitin 4-sulfate-like components were found to be present in smaller amounts, even in samples derived from tumor cells cultured in vitro. A component exhibiting a keratan sulfate-like behavior was detected in fractions obtained from the solid CXBG, CXBI and Furth tumors.
使用微电泳、特定粘多糖酶降解、纸色谱法以及在某些情况下使用离子交换色谱法，对四种小鼠肥大细胞瘤（AB-CBF1-MCT-1、AB-CXBG-MCT-1、AB-CXBI-MCT-1和Furth）的糖胺聚糖组分进行了分离和表征。使用了肌肉内和腹水肿瘤以及体外培养的肿瘤。糖胺聚糖用[35S]硫酸盐标记，在少数情况下用D-[6-3H]葡糖胺标记。从体外培养的AB-CBF1-MCT-1肥大细胞瘤获得的糖胺聚糖组分主要由硫酸皮肤素样物质组成。另一方面，观察到AB-CXBG-MCT-1、AB-CXBI-MCT-1和Furth肿瘤的糖胺聚糖组分的主要成分表现得像肝素或硫酸乙酰肝素。在大多数检查的肥大细胞瘤样本中，发现硫酸软骨素4-硫酸盐样成分含量较少，即使在来自体外培养肿瘤细胞的样本中也是如此。在从实体CXBG、CXBI和Furth肿瘤获得的组分中检测到一种表现出硫酸角质素样行为的成分。
