安替比林血浆半衰期。恒河猴（猕猴）氧化代谢能力的体内指标。

Antipyrine plasma half-life. In vivo indicator of oxidative metabolic capability in Rhesus monkeys (Macaca mulatta).

作者信息

Miller J L, Clark C R, Gee S J, Krieger R I

出版信息

Pharmacology. 1978;16(5):279-86. doi: 10.1159/000136780.

Abstract

Antipyrine plasma half-life (APH) has been used as an indicator of hepatic oxidase activity in rhesus monkeys (Macaca mulatta). Plasma disappearance of 14CH3-N-antipyrine (AP) was measured radiometrically. AP and 3 metabolites were detected using high pressure liquid chromatography. APH was assessed during a 300-day control period and following phenobarbital (PB) pretreatments. Significant interindividual variability was observed requiring that animals be used as their own controls. PB (15 mg/kg i.m., 2/day X 4 days) reduced mean APH values from 81 +/- 12 to 41 +/- 2 min. PB treatment also increased monooxygenase catalyzed aldrin epoxidation, dihydroisodrin hydroxylation and benzo(a)pyrene oxidation measured using liver biopsy homogenates. These parameters permit 'continual' assessment of HMO activity in rhesus monkeys under a variety of experimental conditions.

摘要

安替比林血浆半衰期（APH）已被用作恒河猴（猕猴）肝脏氧化酶活性的指标。采用放射性测量法测定了14CH3-N-安替比林（AP）的血浆消失情况。使用高压液相色谱法检测了AP及其3种代谢产物。在300天的对照期以及苯巴比妥（PB）预处理后评估了APH。观察到显著的个体间变异性，这要求将动物自身作为对照。PB（15毫克/千克，肌肉注射，每天2次，共4天）使平均APH值从81±12分钟降至41±2分钟。PB处理还增加了使用肝活检匀浆测定的单加氧酶催化的艾氏剂环氧化、异狄氏剂二醇羟基化和苯并(a)芘氧化。这些参数允许在各种实验条件下对恒河猴的肝微粒体氧化酶（HMO）活性进行“连续”评估。