核糖核酸酶对细菌和动物细胞的多核糖体及核糖体的作用。
The effect of ribonuclease on polysomes and ribosomes of bacteria and animal cells.
作者信息
Fenwick M L
出版信息
Biochem J. 1968 Apr;107(4):481-9. doi: 10.1042/bj1070481.
Abstract
The mildest treatment with ribonuclease that causes any disaggregation of the polysomes of Escherichia coli or HeLa cells simultaneously attacks the RNA of the constituent ribosomes. It is concluded that the susceptibility to ribonuclease of polysomes does not suggest that they are held together by a strand of messenger RNA. The RNA of the larger sub-unit of bacterial ribosomes has particularly sensitive regions resulting in a non-random degradation. The RNA of the smaller sub-unit of E. coli ribosomes is relatively resistant to ribonuclease attack. The same may be true of the respective sub-units of the intact HeLa-cell ribosome, but both sub-units become very sensitive to ribonuclease on dissociation from each other.
摘要
用核糖核酸酶进行的最温和处理，只要能引起大肠杆菌或海拉细胞多核糖体的任何解聚，就会同时攻击组成核糖体的RNA。由此得出结论，多核糖体对核糖核酸酶的敏感性并不表明它们是由一条信使RNA链维系在一起的。细菌核糖体较大亚基的RNA有特别敏感的区域，导致非随机降解。大肠杆菌核糖体较小亚基的RNA对核糖核酸酶的攻击相对有抗性。完整的海拉细胞核糖体的各个亚基可能也是如此，但两个亚基一旦彼此解离，就会变得对核糖核酸酶非常敏感。
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