大肠杆菌核糖体中核糖核酸的构象。来自核糖核酸酶II作用模式的推断。
The conformation of ribonucleic acids in Escherichia coli ribosomes. Inferences from the mode of action of ribonuclease II.
作者信息
Artman M, Silman N, Engelberg H
出版信息
Biochem J. 1967 Sep;104(3):878-87. doi: 10.1042/bj1040878.
Abstract
- Ribonuclease II of Escherichia coli degrades pulse-labelled RNA associated with ribosomes and polyuridylic acid on ribosomes and in solution to mononucleotides. 2. Ribosomal and pulse-labelled RNA in solution and ribosomal RNA in chloramphenicol particles (protein-deficient ribosomes) are degraded to oligonucleotides. 3. Ribosomal RNA in mature ribosomes is not attacked by the enzyme. 4. From the mode of action of ribonuclease II, which is specific for single-stranded polyribonucleotides and does not attack helical forms, it is inferred that pulse-labelled RNA associated with ribosomes of E. coli exists as a single-stranded structure and that ribosomal RNA in chloramphenicol particles has a pronounced helical character. 5. The different behaviour of ribonuclease II towards newly synthesized RNA, ribosomal RNA and chloramphenicol-particle RNA in E. coli ribosomes is discussed.
摘要
- 大肠杆菌的核糖核酸酶II将与核糖体相关的脉冲标记RNA以及核糖体上和溶液中的聚尿苷酸降解为单核苷酸。2. 溶液中的核糖体RNA和脉冲标记RNA以及氯霉素颗粒（蛋白质缺陷型核糖体）中的核糖体RNA被降解为寡核苷酸。3. 成熟核糖体中的核糖体RNA不会受到该酶的攻击。4. 核糖核酸酶II对单链多聚核糖核苷酸具有特异性且不攻击螺旋形式，从其作用方式可以推断，与大肠杆菌核糖体相关的脉冲标记RNA以单链结构存在，并且氯霉素颗粒中的核糖体RNA具有明显的螺旋特征。5. 讨论了核糖核酸酶II对大肠杆菌核糖体中新合成的RNA、核糖体RNA和氯霉素颗粒RNA的不同作用。
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