康宁木霉纤维素酶系统的亲和层析
Affinity chromatography of the cellulase system of Trichoderma koningii.
作者信息
Halliwell G, Griffin M
出版信息
Biochem J. 1978 Mar 1;169(3):713-5. doi: 10.1042/bj1690713.
Abstract
A procedure involving affinity chromatography on cellulose was developed for separating enzymic components of the cellulase complex. Cellobiase, carboxymethylcellulase, component C2 and cellobiohydrolase are adsorbed with increasing tenacity, and released, as highly purified components, as the ionic strength of the eluent is decreased.
摘要
开发了一种涉及在纤维素上进行亲和色谱的方法，用于分离纤维素酶复合物的酶组分。纤维二糖酶、羧甲基纤维素酶、组分C2和纤维二糖水解酶的吸附力逐渐增强，并随着洗脱液离子强度的降低，作为高度纯化的组分被释放出来。
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本文引用的文献
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HYDROLYSIS OF FIBROUS COTTON AND REPRECIPITATED CELLULOSE BY CELLULOLYTIC ENZYMES FROM SOIL MICRO-ORGANISMS.土壤微生物来源的纤维素酶对纤维状棉花和再生纤维素的水解作用
Biochem J. 1965 Apr;95(1):270-81. doi: 10.1042/bj0950270.
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The action of cellulolytic enzymes from Myrothecium verrucaria.疣孢漆斑菌纤维素分解酶的作用
Biochem J. 1961 Apr;79(1):185-92. doi: 10.1042/bj0790185.
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Individual roles of cellulase components derived from Trichoderma viride.源自绿色木霉的纤维素酶组分的各自作用。
Arch Biochem Biophys. 1965 Aug;111(2):439-47. doi: 10.1016/0003-9861(65)90207-9.
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The formation of short fibres from native cellulose by components of Trichoderma koningii cellulase.康宁木霉纤维素酶组分将天然纤维素转化为短纤维。
Biochem J. 1970 Jan;116(1):35-42. doi: 10.1042/bj1160035.
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Interactions between components of the cellulase complex of Trichoderma koningii on native substrates.康宁木霉纤维素酶复合体各组分在天然底物上的相互作用。
Arch Mikrobiol. 1971;78(4):295-309. doi: 10.1007/BF00412270.
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The nature and mode of action of the cellulolytic component C1 of Trichoderma koningii on native cellulose.康宁木霉纤维素分解成分C1对天然纤维素的作用性质及作用方式
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