Harold F M, Baarda J R
J Bacteriol. 1968 Dec;96(6):2025-34. doi: 10.1128/jb.96.6.2025-2034.1968.
We studied the effect of compounds that uncouple oxidative phosphorylation on membrane function in Streptoccocus faecalis, an organism which relies upon glycolysis for the generation of metabolic energy. At low concentrations (ranging from 10(-7) to 10(-4)m), tetrachlorosalicylanilide, tetramethyldipicrylamine, carbonylcyanide m-chlorophenylhydrazone, pentachlorophenol, and dicoumarol strongly inhibited energy-dependent transport of rubidium, phosphate, and certain amino acids. However, these compounds had little effect on the generation of adenosine triphosphate via glycolysis or on its utilization for the synthesis of macromolecules. They also did not seriously inhibit uptake of those monosaccharides and amino acids which do not require concurrent metabolism. It is proposed that the uncouplers interfere with the utilization of metabolic energy for membrane transport. The uncouplers accelerated the translocation of protons across the cytoplasmic membrane. It appears that a proton-impermeable membrane is required for transport, perhaps, because a proton gradient is involved in the coupling of metabolic energy to the translocation of substrates across the membrane.
我们研究了使氧化磷酸化解偶联的化合物对粪链球菌膜功能的影响，粪链球菌是一种依靠糖酵解产生代谢能量的生物体。在低浓度（范围从10⁻⁷到10⁻⁴m）时，四氯水杨酰苯胺、四甲基二苦胺、羰基氰化物间氯苯腙、五氯苯酚和双香豆素强烈抑制铷、磷酸盐和某些氨基酸的能量依赖性转运。然而，这些化合物对通过糖酵解产生三磷酸腺苷或其用于大分子合成的利用影响很小。它们也没有严重抑制那些不需要同时进行代谢的单糖和氨基酸的摄取。有人提出，解偶联剂会干扰代谢能量用于膜转运的过程。解偶联剂加速了质子穿过细胞质膜的转运。似乎运输需要质子不可渗透的膜，也许是因为质子梯度参与了代谢能量与底物跨膜转运的偶联。
