大肠杆菌中质子偶联的D-木糖转运
Proton-linked D-xylose transport in Escherichia coli.
作者信息
Lam V M, Daruwalla K R, Henderson P J, Jones-Mortimer M C
出版信息
J Bacteriol. 1980 Jul;143(1):396-402. doi: 10.1128/jb.143.1.396-402.1980.
Abstract
The addition of xylose to energy-depleted cells of Escherichia coli elicited an alkaline pH change which failed to appear in the presence of uncoupling agents. Accumulation of [14C]xylose by energy-replete cells was also inhibited by uncoupling agents, but not by fluoride or arsenate. Subcellular vesicles of E. coli accumulated [14C]xylose provided that ascorbate plus phenazine methosulfate were present for respiration, and this accumulation was inhibited by uncoupling agents or valinomycin. Therefore, the transport of xylose into E. coli appears to be energized by a proton-motive force, rather than by a phosphotransferase or directly energized mechanism. Its specificity for xylose as inducer and substrate and the genetic location of a xylose-H+ transport-negative mutation near mtl showed that the xylose-H+ system is distinct from other proton-linked sugar transport systems of E. coli.
摘要
向能量耗尽的大肠杆菌细胞中添加木糖会引起碱性pH变化，而在解偶联剂存在的情况下这种变化不会出现。能量充足的细胞对[14C]木糖的积累也会受到解偶联剂的抑制，但不受氟化物或砷酸盐的抑制。只要存在抗坏血酸盐和吩嗪甲硫酸盐用于呼吸作用，大肠杆菌的亚细胞囊泡就能积累[14C]木糖，并且这种积累会受到解偶联剂或缬氨霉素的抑制。因此，木糖进入大肠杆菌的转运似乎是由质子动力提供能量，而不是由磷酸转移酶或直接供能机制提供能量。它对木糖作为诱导剂和底物的特异性以及木糖-H+转运阴性突变在mtl附近的基因定位表明，木糖-H+系统与大肠杆菌的其他质子偶联糖转运系统不同。
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