荧光密螺旋体抗体吸收试验中非特异性背景染色的减少。
Reduction of nonspecific background staining in the fluorescent treponemal antibody-absorption test.
作者信息
Roberts M E, Miller J N, Binnings G F
出版信息
J Bacteriol. 1968 Nov;96(5):1500-6. doi: 10.1128/jb.96.5.1500-1506.1968.
Abstract
The nonspecific background fluorescence which occurs with the fluorescent treponemal antibody-absorption test for syphilis was found to result from a reaction between serum-treated Treponema pallidum organisms and the conjugated antihuman gamma-globulin. It was also shown that beta-lipoprotein and albumin were the important contributing factors in human serum. Various dilutions of 2.5% trypsin in phosphate-buffered saline specifically reduced background fluorescence under proper test conditions. By employing a trypsin digestion method, a semiautomated procedure utilizing a visual readout has been postulated as feasible.
摘要
梅毒荧光密螺旋体抗体吸收试验中出现的非特异性背景荧光，被发现是血清处理的梅毒螺旋体生物体与共轭抗人γ球蛋白之间反应的结果。还表明，β脂蛋白和白蛋白是人类血清中的重要促成因素。在适当的试验条件下，2.5%胰蛋白酶在磷酸盐缓冲盐水中的各种稀释液可特异性降低背景荧光。通过采用胰蛋白酶消化方法，一种利用视觉读数的半自动程序被认为是可行的。
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