Kirkpatrick J B
Science. 1969 Jan 10;163(3863):187-8. doi: 10.1126/science.163.3863.187.
Microtubules from neurons are preserved in homogenates of mammalian brain by medium containing organic solvents at acidic pH. By means of negative staining and electron microscopy, the relative concentration of microtubules in suspensions can be assayed. Microtubules from brain have a filamentous and substructure.
来自神经元的微管在酸性pH值下通过含有有机溶剂的培养基保存在哺乳动物脑匀浆中。通过负染色和电子显微镜,可以测定悬浮液中微管的相对浓度。来自脑的微管具有丝状和亚结构。