Drijver J S, Boon M E
Acta Cytol. 1983 Nov-Dec;27(6):693-8.
The role of phosphotungstic acid as the main pH-controlling agent in Papanicolaou's EA stain was analyzed, including its effect on the competition between eosin Y and light green, the principal components of EA solutions, and this acid was compared with HCl as an acidifying agent. The amount of phosphotungstic acid in the solution determined the color of the cytoplasm. Our study led to a better understanding and a simplification of the staining procedure. With the acquired knowledge, the Papanicolaou counterstain can be standardized to suit the taste of the individual cytologist and can add to the predictability of the results.
分析了磷钨酸在巴氏EA染色中作为主要pH调节剂的作用，包括其对EA溶液主要成分伊红Y和亮绿之间竞争的影响，并将该酸与作为酸化剂的盐酸进行了比较。溶液中磷钨酸的量决定了细胞质的颜色。我们的研究有助于更好地理解和简化染色程序。凭借所获得的知识，巴氏复染可以标准化，以适应个体细胞学家的喜好，并提高结果的可预测性。
