Tickner T R, Gutteridge J M
Clin Chim Acta. 1978 Apr 17;85(2):125-9. doi: 10.1016/0009-8981(78)90231-0.
A new colorimetric method for the assay of biliverdin in biological fluids is described. The method, based upon the reaction of biliverdin with barbituric acid, offers improved sensitivity and selectivity when compared to direct spectrophotometric measurements. Using this method biliverdinaemia was observed in two patients with obstructive jaundice of malignant origin.
本文描述了一种用于测定生物体液中胆绿素的新比色法。该方法基于胆绿素与巴比妥酸的反应,与直接分光光度法相比,具有更高的灵敏度和选择性。使用该方法在两名恶性梗阻性黄疸患者中观察到了胆绿素血症。