The forensic identification of semen by isoelectric focusing of seminal acid phosphatase.

An isoelectric focusing technique for the separation of acid phosphatases is described. The focusing patterns for semen and vaginal secretion are examined in detail and the technique is evaluated as a means of identifying and distinguishing between these body fluids and other materials of potential forensic interest. The method can reliably be used to diagnose the presence of semen in stains and in the post-coital vagina.