Stolorow M D, Hauncher J D, Stuver W C
J Assoc Off Anal Chem. 1976 Nov;59(6):1352-6.
Recent advances in forensic science in the identification of human seminal acid phosphatase are presented, with particular attention to the acrylamide gel electrophoretic method. In that method a difficulty in distinguishing seminal acid phosphatase from certain fecal phosphatases has been observed and an attempt is made here to distinguish the phosphatases from one another experimentally by differential substrate specificity. 4-Methylumbelliferyl phosphate and alpha-naphthyl acid phosphate are used as reaction substrates. Although seminal and vaginal acid phosphatases are differentiated by the modified method, fecal phosphatase is not clearly differentiated from seminal acid phosphatase.
本文介绍了法医学在鉴定人类精浆酸性磷酸酶方面的最新进展，尤其关注丙烯酰胺凝胶电泳法。在该方法中，已观察到区分精浆酸性磷酸酶与某些粪便磷酸酶存在困难，本文试图通过不同的底物特异性进行实验，以区分这些磷酸酶。4-甲基伞形酮磷酸酯和α-萘基磷酸用作反应底物。虽然改良方法可区分精浆和阴道酸性磷酸酶，但粪便磷酸酶与精浆酸性磷酸酶无法明确区分。
