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葡聚糖凝胶G - 200凝胶过滤在血型抗体分析中的价值

Value of gel filtration on Sephadex G-200 in the analysis of blood group antibodies.

作者信息

Stratton F, Smith D S, Rawlinson V I

出版信息

J Clin Pathol. 1968 Nov;21(6):708-14. doi: 10.1136/jcp.21.6.708.

DOI:10.1136/jcp.21.6.708
PMID:4180468
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC473944/
Abstract

Analysis of blood group antibodies on Sephadex G-200 columns is described. The position of blood group antibodies when whole serum containing them is subjected to gel filtration is studied and compared with the position of the gamma-globulins and of standard blood group antibodies. The technique on its own cannot be used to identify the immunoglobulin nature of the antibody but is very useful when employed in conjunction with the antiglobulin test using specific antisera and with other methods. The technique is rapid, simple, and reliable.

摘要

本文描述了在Sephadex G - 200柱上对血型抗体的分析。研究了含有血型抗体的全血清进行凝胶过滤时血型抗体的位置,并与γ - 球蛋白和标准血型抗体的位置进行比较。该技术本身不能用于鉴定抗体的免疫球蛋白性质,但与使用特异性抗血清的抗球蛋白试验及其他方法联合使用时非常有用。该技术快速、简单且可靠。

相似文献

1
Value of gel filtration on Sephadex G-200 in the analysis of blood group antibodies.葡聚糖凝胶G - 200凝胶过滤在血型抗体分析中的价值
J Clin Pathol. 1968 Nov;21(6):708-14. doi: 10.1136/jcp.21.6.708.
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本文引用的文献

1
QUANTITATIVE DETERMINATION OF SERUM IMMUNOGLOBULINS IN ANTIBODY-AGAR PLATES.抗体琼脂平板中血清免疫球蛋白的定量测定
J Immunol. 1965 Jan;94:84-90.
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IMMUNOCHEMICAL STUDIES OF THE RH SYSTEM. I. ISOLATION AND CHARACTERIZATION OF ANTIBODIES.RH系统的免疫化学研究。I. 抗体的分离与特性鉴定
J Immunol. 1964 May;92:682-92.
3
SEROLOGICAL CHARACTERISTICS OF ANTI-A RELATED TO TYPE OF ANTIBODY PROTEIN (7 S GAMMA OR 19 S GAMMA).与抗体蛋白类型(7Sγ或19Sγ)相关的抗A血清学特征
Vox Sang. 1963 Jul-Aug;8:385-409. doi: 10.1111/j.1423-0410.1963.tb04159.x.
4
Fractionation of human blood group antibodies by gel filtration.通过凝胶过滤法分离人类血型抗体。
Scand J Clin Lab Invest. 1963;15 Suppl 69:130-8.
5
The preparation and uses of antiglobulin reagents with special reference to complement-fixing blood group antibodies.抗球蛋白试剂的制备及用途,特别涉及补体结合血型抗体
Transfusion. 1962 May-Jun;2:135-49. doi: 10.1111/j.1537-2995.1962.tb00212.x.
6
Anticomplementary activity of sera from patients with connective tissue disease and normal subjects.
J Lab Clin Med. 1967 Feb;69(2):217-28.
7
Gamma-A-blood group antibodies.γ-A血型抗体
J Exp Med. 1966 May 1;123(5):951-67. doi: 10.1084/jem.123.5.951.
8
Immunochemical properties of human gamma-A isohemagglutinin. I. Comparisons with gamma-G and gamma-M-globulin antibodies.人γ-A 同种血凝素的免疫化学特性。I. 与γ-G 和γ-M 球蛋白抗体的比较。
J Immunol. 1965 Aug;95(2):197-208.