Pearson C M, Wood F D
Immunology. 1969 Feb;16(2):157-65.
The addition of 10 mg of ovalbumin (HEA), bovine γ-globulin (BGG) or rat γ-globulin (RGG) to a mycobacterial wax D adjuvant emulsion inoculated intracutaneously reduced the induction of adjuvant arthritis from a 95 per cent incidence in control rats to 18.5, 26.6 and 33 per cent, respectively. There was notably less protection from bovine plasma albumin, rat serum albumin or heat denatured rat serum albumin. With a 5-mg dose of HEA arthritis was reduced to 37.5 per cent. Lower dosages were not protective. Little or no protection resulted from pre-treatments with HEA or BGG intramuscularly, with BGG in incomplete adjuvant intracutaneously or from the latter given separately but concurrently with wax D adjuvant. The inhibitory effects of the various proteins may be attributed to their adsorption by wax D before inoculation. Alternatively the protein may compete as an immunogen with an immunogenic moiety of the wax D.
将10毫克卵清蛋白（HEA）、牛γ球蛋白（BGG）或大鼠γ球蛋白（RGG）添加到皮内接种的分枝杆菌蜡D佐剂乳剂中，可将佐剂性关节炎的诱导率从对照大鼠中的95%分别降至18.5%、26.6%和33%。牛血浆白蛋白、大鼠血清白蛋白或热变性大鼠血清白蛋白提供的保护明显较少。使用5毫克剂量的HEA时，关节炎发生率降至37.5%。较低剂量无保护作用。肌肉注射HEA或BGG、皮内注射不完全佐剂中的BGG或单独但与蜡D佐剂同时给予BGG进行预处理，几乎没有或没有产生保护作用。各种蛋白质的抑制作用可能归因于接种前它们被蜡D吸附。或者，蛋白质可能作为免疫原与蜡D的免疫原性部分竞争。
