Vernon-Roberts B, Liyanage S P, Currey H L
Ann Rheum Dis. 1975 Oct;35(5):389-97. doi: 10.1136/ard.35.5.389.
Adjuvant disease in the rat may represent a cell-mediated response to tuberculous material disseminated from the original injection site, but previous studies have provided only indirect evidence for this dissemination. In the present experiments tubercle bacilli labelled in vitro with rhodamine isothiocyanate (RITC) were injected into a footpad as Freund's complete adjuvant. Serial studies showed two varieties of fluorescent material in the tissues (1) intracellular and extracellular intact and fragmented bacilli, and (2) amorphous intracellular material. Both types of material were identified in the injected foot and draining lymph nodes. Bacilli were also identified in the contralateral knee joint, peritoneum, pleura, lung, and liver, while amorphous material alone appeared in the spleen. The presence of intact bacilli was confirmed by positive Ziehl-Nielsen staining of the organisms, but the amorphous intracellular material did not stain positively by this method. The use of RITC-labelled organisms considerably reduced the severity of adjuvant disease. Most of the organisms identified in sites distant from the injected limb were not situated within foci of inflammation. Marked differences in processing of the tuberculous material (and lack of dissemination of intact bacilli) were noted when labelled organisms were injected in saline instead of in oil.
大鼠的佐剂病可能代表对从最初注射部位扩散的结核物质的细胞介导反应，但先前的研究仅提供了这种扩散的间接证据。在本实验中，将用异硫氰酸罗丹明（RITC）体外标记的结核杆菌作为弗氏完全佐剂注入足垫。系列研究显示组织中有两种荧光物质：（1）细胞内和细胞外完整及破碎的杆菌，以及（2）无定形细胞内物质。在注射侧足和引流淋巴结中均鉴定出这两种物质。在对侧膝关节、腹膜、胸膜、肺和肝脏中也鉴定出了杆菌，而脾脏中仅出现了无定形物质。通过对菌体进行齐-尼氏染色阳性证实了完整杆菌的存在，但这种无定形细胞内物质用此方法染色呈阴性。使用RITC标记的菌体大大降低了佐剂病的严重程度。在远离注射肢体的部位鉴定出的大多数菌体并不位于炎症灶内。当将标记的菌体注入盐水中而非油中时，发现结核物质的处理存在明显差异（且完整杆菌未扩散）。
