Majerfeld I, Barlati S, Ciferri O
J Bacteriol. 1970 Feb;101(2):350-4. doi: 10.1128/jb.101.2.350-354.1970.
A decline in colony-forming ability is observed in actively growing cultures of a tryptophan arginine auxotroph of Bacillus subtilis after removal of tryptophan (tryptophanless death). This phenomenon can be prevented by simultaneous starvation of the other required amino acid or by chloramphenicol administered in bacteriostatic concentration but not by actinomycin. Addition of tryptophan analogues not only prevents the death but also allows recovery of the cells that have lost the ability to form colonies on solid media. The term tryptophanless death is therefore inappropriate. Chloramphenicol but not actinomycin inhibits the recovery brought about by tryptophan analogues.
在去除色氨酸后，枯草芽孢杆菌色氨酸精氨酸营养缺陷型的活跃生长培养物中观察到集落形成能力下降（色氨酸缺乏死亡）。这种现象可以通过同时饥饿其他必需氨基酸或通过以抑菌浓度施用氯霉素来预防，但不能通过放线菌素预防。添加色氨酸类似物不仅可以防止细胞死亡，还能使在固体培养基上失去形成菌落能力的细胞恢复。因此，“色氨酸缺乏死亡”这一术语并不恰当。氯霉素而非放线菌素会抑制色氨酸类似物带来的细胞恢复。
