Barlati S, Ciferri O
J Bacteriol. 1970 Jan;101(1):166-72. doi: 10.1128/jb.101.1.166-172.1970.
Addition of the tryptophan analogues 5-methyl-tryptophan, 5-hydroxy-tryptophan, and 7-aza-tryptophan, during tryptophan starvation of a tryptophan auxotroph of Bacillus subtilis, stimulated amino acid incorporation. Two of the analogues tested, 5-methyl-tryptophan and 5-hydroxy-tryptophan, competed with each other, but their addition did not prevent tryptophan incorporation into acid-insoluble material. The incorporation of tryptophan and 5-hydroxy-tryptophan was stimulated when a mixture of phenylalanine and tyrosine was present in the medium. The two analogues 5-methyl-tryptophan and 5-hydroxy-tryptophan were recovered in protein hydrolysates of analogue-grown cultures, and there is evidence suggesting that the analogues are not chain terminators. Neither analogue prevented the polysome degradation that occurs during tryptophan starvation.
在枯草芽孢杆菌色氨酸营养缺陷型菌株色氨酸饥饿期间，添加色氨酸类似物5-甲基色氨酸、5-羟色氨酸和7-氮杂色氨酸可刺激氨基酸掺入。所测试的两种类似物，即5-甲基色氨酸和5-羟色氨酸，相互竞争，但它们的添加并不妨碍色氨酸掺入酸不溶性物质中。当培养基中存在苯丙氨酸和酪氨酸的混合物时，色氨酸和5-羟色氨酸的掺入受到刺激。在类似物培养的培养物的蛋白质水解物中回收了5-甲基色氨酸和5-羟色氨酸这两种类似物，并且有证据表明这些类似物不是链终止剂。这两种类似物都不能阻止色氨酸饥饿期间发生的多核糖体降解。
