Correa W M
Can J Comp Med. 1970 Apr;34(2):164-6.
Smears and imprints were made from the liver of 27 equine fetuses, believed to have aborted as a result of Equine Virus Abortion (EVA) infection. Several different fixatives and staining techniques were employed for the demonstration of typical intra-nuclear inclusion bodies in these preparations, and the following conclusions were reached. Methanol proved to be the best fixative and Pappenheim's panoptic method was the best staining technique, giving good contrast and definition of the inclusion bodies. Cytological methods provided a simple and rapid means of diagnosis, but histological sections provided evidence of lesions which was most useful when inclusion bodies were very difficult to find. However, cytological methods proved better than histological sections for the demonstration of EVA intranuclear inclusion bodies.
对27匹疑似因马病毒性流产（EVA）感染而流产的马胎儿的肝脏制作涂片和压片标本。采用了几种不同的固定剂和染色技术来显示这些标本中的典型核内包涵体，并得出以下结论。甲醇被证明是最佳固定剂，帕彭海姆全染法是最佳染色技术，能使包涵体呈现出良好的对比度和清晰度。细胞学方法提供了一种简单快速的诊断手段，但组织学切片能提供病变证据，在包涵体极难发现时最为有用。然而，在显示EVA核内包涵体方面，细胞学方法被证明比组织学切片更好。
