碳水化合物与骨髓瘤免疫球蛋白轻链可变区的连接。
Attachment of carbohydrate to the variable region of myeloma immunoglobulin light chains.
作者信息
Sox H C, Hood L
出版信息
Proc Natl Acad Sci U S A. 1970 Jul;66(3):975-82. doi: 10.1073/pnas.66.3.975.
Abstract
Approximately 15 per cent of the light chains from homogeneous immunoglobulins in patients with multiple myeloma contain an oligosaccharide group. Five human myeloma light chains that contained carbohydrate were studied. The sequence Asn-[unk]-Ser/Thr was at the site of carbohydrate attachment in all light chains. The carbohydrate group was attached to the asparagine residue of this triplet sequence which in all five light chains was located in the variable region. The occasional occurrence of carbohydrate in myeloma light chains is seen as the consequence of a variable region mutation creating an Asn-[unk]-Ser/Thr sequence to which carbohydrate is attached by an enzyme capable of recognizing the characteristic triplet sequence.
摘要
多发性骨髓瘤患者中，约15%的同源免疫球蛋白轻链含有一个寡糖基团。对五条含有碳水化合物的人骨髓瘤轻链进行了研究。在所有轻链中，碳水化合物连接位点的序列为天冬酰胺-[未知]-丝氨酸/苏氨酸。碳水化合物基团连接到该三联体序列的天冬酰胺残基上，在所有五条轻链中，该残基都位于可变区。骨髓瘤轻链中偶尔出现碳水化合物被视为可变区突变的结果，该突变产生了一个天冬酰胺-[未知]-丝氨酸/苏氨酸序列，一种能够识别该特征三联体序列的酶将碳水化合物连接到该序列上。
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