Soll D R
Science. 1979 Mar 2;203(4383):841-9. doi: 10.1126/science.419408.
Conditional methods are proposed for investigating the number and relationships of processes that are rate-limiting for the genesis of consecutive stages in a developmental sequence. These methods depend on the differential sensitivity of "timer" pathways to small changes in temperature and can be applied to any developmental sequence in which discrete stages can be reproducibly monitored with time. We have applied the methods to multicellular morphogenesis in the slime mold Dictyostelium discoideum and have obtained an unexpected tentative scheme for timer relationships. A minimum of six timers has been delineated, each specific for at least one morphological stage. The majority of these timers appear to be in parallel.
我们提出了条件方法来研究发育序列中连续阶段发生过程中的限速过程数量及其关系。这些方法依赖于“计时”途径对温度微小变化的差异敏感性，并且可以应用于任何能够随时间可重复监测离散阶段的发育序列。我们已将这些方法应用于黏菌盘基网柄菌的多细胞形态发生，并得到了一个关于计时关系的意外初步方案。已确定至少六个计时，每个计时至少对应一个形态阶段。这些计时中的大多数似乎是并行的。
