Flores F, Naftolin F, Ryan K J, White R J
Science. 1973 Jun 8;180(4090):1074-5. doi: 10.1126/science.180.4090.1074.
Perfusion of two isolated brains from immature male rhesus monkeys with [(3)H]androstenedione resulted in the identification of free and conjugated [(3)H]estrone and free [(3)H]estradiol from the perfusates. In the dissected cerebral tissues, estrogens were recovered only from the hypothalamus and limbic system. The production of estrogens from androstenedione during the 40-minute perfusions in these two experiments totaled 1.58 and 2.83 nanograms.
用[³H]雄烯二酮灌注两只未成熟雄性恒河猴的离体大脑，结果从灌注液中鉴定出游离和结合的[³H]雌酮以及游离的[³H]雌二醇。在解剖的脑组织中，仅在下丘脑和边缘系统中检测到雌激素。在这两个实验中，40分钟灌注期间雄烯二酮产生的雌激素总量分别为1.58纳克和2.83纳克。
