Pfaff D W, Gerlach J L, McEwen B S, Ferin M, Carmel P, Zimmerman E A
J Comp Neurol. 1976 Dec 1;170(3):279-93. doi: 10.1002/cne.901700302.
With autoradiographic procedures, cells which bind 3H-estradiol were found in preoptic, hypothalamic and limbic structures in the brains of ovariectomized, adult female rhesus monkeys. Estrogen-binding cells were seen in the medial preoptic area, medial anterior hypothalamus, ventromedial nucleus, and especially heavy labelling was seen throughout the extent of the arcuate (infundibular) nucleus of the hypothalamus. In limbic structures, cells in the bed nucleus of the stria terminalis and in the medial nucleus of the amygdala were well labelled. Systematic charting also revealed smaller numbers of estrogen-concentrating cells in other specific hypothalamic and limbic locations. In the anterior pituitary, significant numbers of basophils and acidophils were found to bind estrogen. Pars intermedia and the posterior lobe were virtually unlabelled. In the uterus, heavily labelled cells were seen in the endometrial stroma and in the myometrium. These autoradiographic findings agree with results of parallel biochemical experiments. In monkeys injected with 3H-corticosterone, the most extensive high-intensity binding found with autoradiography was in the hippocampus. Both pyramidal neurons and dentate gyrus granule cells were labelled. Biochemical experiments, also, showed highest cell nuclear accumulation of corticosterone in the hippocampus. Findings with estradiol in the rhesus monkey extend to primates conclusions based on autoradiographic experiments with steroid sex hormones in wide variety of vertebrates, including fish, amphibians, birds, and various mammalian species (Morell st al., '75a). All of these vertebrate forms have sex hormone-concentrating neurons, which are found in specific preoptic, hypothalamic and limbic structures. In the species studied, such hormone-concentrating neurons appear to be involved in the hormonal control of behavioral and pituitary function.
采用放射自显影术，在成年去卵巢雌性恒河猴大脑的视前区、下丘脑和边缘系统结构中发现了能结合³H-雌二醇的细胞。在视前内侧区、下丘脑前内侧、腹内侧核中可见雌激素结合细胞，而下丘脑弓状（漏斗）核全程标记尤为明显。在边缘系统结构中，终纹床核和杏仁核内侧核中的细胞标记良好。系统绘图还显示，在其他特定的下丘脑和边缘系统位置也有少量雌激素聚集细胞。在前垂体中，发现大量嗜碱性细胞和嗜酸性细胞能结合雌激素。中间部和后叶几乎没有标记。在子宫中，子宫内膜基质和子宫肌层中可见标记明显的细胞。这些放射自显影结果与平行生化实验结果一致。给猴子注射³H-皮质酮后，放射自显影发现结合最广泛且强度最高的部位是海马体。锥体细胞和齿状回颗粒细胞均有标记。生化实验也表明，海马体中皮质酮的细胞核积累量最高。恒河猴中关于雌二醇的研究结果，将基于对包括鱼类、两栖类、鸟类和各种哺乳动物在内的多种脊椎动物进行的类固醇性激素放射自显影实验得出的结论扩展到了灵长类动物（莫雷尔等人，1975a）。所有这些脊椎动物都有性激素聚集神经元，它们存在于特定的视前区、下丘脑和边缘系统结构中。在所研究的物种中，这类激素聚集神经元似乎参与了行为和垂体功能的激素调控。
