Duckworth M, Archibald A R, Baddiley J
Biochem J. 1972 Dec;130(3):691-6. doi: 10.1042/bj1300691.
The N-acetylgalactosamine in the walls of Bacillus subtilis 168 occurs in two polymers. One of these contains N-acetylgalactosamine, glucose and phosphorus and is attached to the peptidoglycan through an alkali-labile bond; preliminary studies indicate that a repeating unit of this polymer is glucosyl-N-acetylgalactosamine 1-phosphate. N-Acetylgalactosamine is also associated with the peptidoglycan in a component that is not converted into the free sugar or other soluble compounds on treatment of the walls with alkali. The two polymers containing N-acetylgalactosamine are released on autolysis of the walls and can be separated by ion-exchange chromatography. As glucose 6-phosphate is produced by gentle hydrolysis of the wall with acid a third phosphate polymer, poly(glucose 1-phosphate), may occur in this wall. However, as no polymer with this structure could be separated from that containing galactosamine, its existence has not been established unequivocally. The methods described permit the study of variations in N-acetylgalactosamine content with respect to growth conditions.
枯草芽孢杆菌168细胞壁中的N-乙酰半乳糖胺存在于两种聚合物中。其中一种含有N-乙酰半乳糖胺、葡萄糖和磷，并通过碱不稳定键连接到肽聚糖上；初步研究表明，这种聚合物的重复单元是葡萄糖基-N-乙酰半乳糖胺1-磷酸。N-乙酰半乳糖胺在一种成分中也与肽聚糖相关，在用碱处理细胞壁时，该成分不会转化为游离糖或其他可溶性化合物。含有N-乙酰半乳糖胺的两种聚合物在细胞壁自溶时释放出来，可通过离子交换色谱法分离。由于用酸对细胞壁进行温和水解会产生6-磷酸葡萄糖，因此这种细胞壁中可能存在第三种磷酸聚合物，即聚（1-磷酸葡萄糖）。然而，由于无法从含有半乳糖胺的聚合物中分离出具有这种结构的聚合物，其存在尚未得到明确证实。所描述的方法允许研究N-乙酰半乳糖胺含量相对于生长条件的变化。
