Hayashi H, Araki Y, Ito E
J Bacteriol. 1973 Feb;113(2):592-8. doi: 10.1128/jb.113.2.592-598.1973.
Analysis by dinitrophenylation techniques revealed the occurrence of significant amounts of glucosamine residues with free amino groups in the peptidoglycan component of cell walls isolated from Bacillus cereus, Bacillus subtilis, and Bacillus megaterium. A close correlation was demonstrated between the content of N-unacetylated glucosamine residues in the peptidoglycan component and the resistance of the cell walls to lysozyme. These lysozyme-resistant cell walls and peptidoglycan were converted into a lysozyme-sensitive form by means of N-acetylation with acetic anhydride. Thus, the occurrence of the N-unacetylated glucosamine residues in the peptidoglycan component accounts for the resistance of these cell walls to lysozyme. The N-unacetylated glucosamine residues were not found in a significant amount in the cell walls of Micrococcus lysodeikticus, Staphylococcus aureus, Streptococcus faecalis, Lactobacillus casei, or Lactobacillus arabinosus.
通过二硝基苯化技术分析发现，从蜡状芽孢杆菌、枯草芽孢杆菌和巨大芽孢杆菌分离出的细胞壁肽聚糖成分中存在大量带有游离氨基的氨基葡萄糖残基。结果表明，肽聚糖成分中N-未乙酰化氨基葡萄糖残基的含量与细胞壁对溶菌酶的抗性之间存在密切相关性。这些抗溶菌酶的细胞壁和肽聚糖通过用乙酸酐进行N-乙酰化转化为溶菌酶敏感形式。因此，肽聚糖成分中N-未乙酰化氨基葡萄糖残基的存在解释了这些细胞壁对溶菌酶的抗性。在溶壁微球菌、金黄色葡萄球菌、粪链球菌、干酪乳杆菌或阿拉伯糖乳杆菌的细胞壁中未发现大量的N-未乙酰化氨基葡萄糖残基。
