铜绿假单胞菌中葡萄糖和α-甲基葡萄糖苷转运的调控
The regulation of transport of glucose and methyl alpha-glucoside in Pseudomonas aeruginosa.
作者信息
Midgley M, Dawes E A
出版信息
Biochem J. 1973 Feb;132(2):141-54. doi: 10.1042/bj1320141.
Abstract
The methyl alpha-glucoside-transport system of Pseudomonas aeruginosa has been characterized with respect to its specificity, energy-dependence, kinetics and regulation. The uptake of glucose involved two components, one of which transported glucose (K(m)=8mum) and methyl alpha-glucoside (K(m)=2.8mm) whereas the other was more complex, involving the extracellular activity of glucose dehydrogenase. Mutants defective in this enzyme have been isolated and characterized. The methyl alpha-glucoside-glucose-transport system was repressed when the organism was grown in the absence of glucose; the induction of this transport system by glucose, and its activity once induced, were inhibited by products of citrate metabolism.
摘要
铜绿假单胞菌的α-甲基葡糖苷转运系统已在特异性、能量依赖性、动力学和调节方面进行了表征。葡萄糖的摄取涉及两个组分，其中一个转运葡萄糖（K(m)=8μm）和α-甲基葡糖苷（K(m)=2.8mm），而另一个则更为复杂，涉及葡萄糖脱氢酶的胞外活性。已分离并表征了该酶缺陷的突变体。当生物体在无葡萄糖的情况下生长时，α-甲基葡糖苷-葡萄糖转运系统受到抑制；该转运系统被葡萄糖诱导，且一旦被诱导，其活性会受到柠檬酸代谢产物的抑制。
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