Collins W P, Collins P O, Kilpatrick M J, Manning P A, Pike J M, Tyler J P
Acta Endocrinol (Copenh). 1979 Feb;90(2):336-48. doi: 10.1530/acta.0.0900336.
Methods based upon the principles of radioimmunoassay have been developed and evaluated for the measurement of oestrone-3-glucuronide, LH and pregnanediol-3alpha-glucuronide in samples of unextracted urine. The procedures have been applied to daily urine (early morning fraction and combined 24 hour collections) from 6 women throughout one complete menstrual cycle and to serial samples from an additional 14 women who only collected early morning specimens. The results showed that there were characteristic, well-defined changes in the concentration of all 3 metabolites in both samples of urine and from all subjects. In addition, there was a reasonable correlation between the concentration of all 3 compounds in samples of early morning urine and the corresponding rates of excretion per 24 hours. These findings suggest that the procedures may be of value for monitoring ovarian function over long periods of time, without the problems of stress and inconvenience to the patient. Furthermore, the ratio of values for oestrone-3-glucuronide to pregnanediol-3alpha-glucuronide may be used to indicate the start and finish of the fertile period.
基于放射免疫分析原理的方法已被开发并评估，用于测量未提取尿液样本中的雌酮 - 3 - 葡萄糖醛酸苷、促黄体生成素（LH）和孕二醇 - 3α - 葡萄糖醛酸苷。这些程序已应用于6名女性在一个完整月经周期内的每日尿液（清晨部分和24小时混合收集），以及另外14名仅收集清晨样本的女性的系列样本。结果表明，在尿液样本和所有受试者中，所有3种代谢物的浓度都有特征性的、明确的变化。此外，清晨尿液样本中所有3种化合物的浓度与每24小时相应的排泄率之间存在合理的相关性。这些发现表明，这些程序对于长期监测卵巢功能可能有价值，而不会给患者带来压力和不便等问题。此外，雌酮 - 3 - 葡萄糖醛酸苷与孕二醇 - 3α - 葡萄糖醛酸苷的值之比可用于指示排卵期的开始和结束。
