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1
Comparison of Bacillus thuringiensis with other bacillus species based on enzymoserological examinations of their proteolytic enzymes.基于对苏云金芽孢杆菌及其它芽孢杆菌属物种的蛋白水解酶进行酶血清学检测的比较研究
Acta Vet Scand. 1973;14(1):176-83. doi: 10.1186/BF03547420.
2
Proteolytic enzymes and biological inhibitors. II. Naturally occurring inhibitors in sera from different species and their effect upon proteolytic enzymes of various origin.
Acta Pathol Microbiol Scand B Microbiol Immunol. 1970;78(5):605-18.
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Proteolytic degradation of polymyxins by the enzymes of Bacillus polymyxa.多粘芽孢杆菌的酶对多粘菌素的蛋白水解降解作用。
Acta Biochim Pol. 1973;20(3):285-96.
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[Studies on the activity of proteolytic enzymes in Bacillus anthracis and Bacillus cereus].[炭疽芽孢杆菌和蜡样芽孢杆菌中蛋白水解酶活性的研究]
Pathol Microbiol (Basel). 1973;39(5):364-72.
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Evidence in favour of the modification in vivo of RNA polymerase subunits during sporogenesis in Bacillus thuringiensis.支持苏云金芽孢杆菌孢子形成过程中RNA聚合酶亚基在体内发生修饰的证据。
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Preparation and purification of microbial proteases with special reference to the bacilli.微生物蛋白酶的制备与纯化，特别提及芽孢杆菌属
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J Allergy Clin Immunol. 1971 Oct;48(4):212-9. doi: 10.1016/0091-6749(71)90067-4.
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本文引用的文献

1
The dissociation of Bacillus thuringiensis.苏云金芽孢杆菌的解离
Can J Microbiol. 1967 Nov;13(11):1566-8. doi: 10.1139/m67-206.
2
Serological studies on proteinases produced by Aeromonas salmonicida and other aeromonads.关于杀鲑气单胞菌及其他气单胞菌所产生蛋白酶的血清学研究。
Acta Vet Scand. 1968;9(1):1-9. doi: 10.1186/BF03547885.
3
Enzymo-serological comparison of proteolytic enzymes produced by salmonella species and other enterobacteria.沙门氏菌属及其他肠道杆菌所产生的蛋白水解酶的酶学血清学比较
Acta Vet Scand. 1969;10(3):225-33. doi: 10.1186/BF03548275.
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Biogenesis of the crystalline inclusion of Bacillus thuringiensis during sporulation.
Eur J Biochem. 1971 Nov 11;23(2):282-94. doi: 10.1111/j.1432-1033.1971.tb01620.x.
5
Enzymoserological relationships between Vibrio comma and other gram-negative organisms.霍乱弧菌与其他革兰氏阴性菌之间的酶血清学关系。
Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(2):285-90. doi: 10.1111/j.1699-0463.1971.tb02156.x.
6
Proteolytic enzymes and biological inhibitors. II. Naturally occurring inhibitors in sera from different species and their effect upon proteolytic enzymes of various origin.
Acta Pathol Microbiol Scand B Microbiol Immunol. 1970;78(5):605-18.
7
Enzymoserological separation of bacterial proteinases.细菌蛋白酶的酶血清学分离
Acta Pathol Microbiol Scand. 1969;77(1):143-8. doi: 10.1111/j.1699-0463.1969.tb04215.x.

基于对苏云金芽孢杆菌及其它芽孢杆菌属物种的蛋白水解酶进行酶血清学检测的比较研究

Comparison of Bacillus thuringiensis with other bacillus species based on enzymoserological examinations of their proteolytic enzymes.

作者信息

Sandvik O

出版信息

Acta Vet Scand. 1973;14(1):176-83. doi: 10.1186/BF03547420.

DOI:10.1186/BF03547420
PMID:4199697
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8559819/
Abstract

Investigated strains of Bacillus thuringiensis were all good producers of casein precipitating proteinases (CP-enzymes). CP-enzymes produced by five strains of B. thuringiensis were serologically compared with enzymes produced by strains of B. cereus, B. licheniformis and B. subtilis. The technique used is a special enzymoserological procedure by which the proteolytic activity is neutralized by specific antisera. A close enzymoserological relationship was demonstrated between B. thuringiensis and B. cereus, while no interspecies cross-reactions occurred between enzymes of these species, and those of B. licheniformis and B. subtilis. The possibility of undetected transference of B. thuringiensis from bacteriologically treated plant material to animals is discussed in relation to the high degree of similarity between B. cereus and B. thuringiensis.

摘要

所研究的苏云金芽孢杆菌菌株都是酪蛋白沉淀蛋白酶（CP-酶）的良好生产者。对五株苏云金芽孢杆菌产生的CP-酶与蜡状芽孢杆菌、地衣芽孢杆菌和枯草芽孢杆菌菌株产生的酶进行了血清学比较。所采用的技术是一种特殊的酶血清学方法，通过该方法，蛋白水解活性可被特异性抗血清中和。结果表明，苏云金芽孢杆菌和蜡状芽孢杆菌之间存在密切的酶血清学关系，而这些物种的酶与地衣芽孢杆菌和枯草芽孢杆菌的酶之间未发生种间交叉反应。鉴于蜡状芽孢杆菌和苏云金芽孢杆菌之间的高度相似性，讨论了经细菌学处理的植物材料中未检测到的苏云金芽孢杆菌转移到动物体内的可能性。