关于杀鲑气单胞菌及其他气单胞菌所产生蛋白酶的血清学研究。
Serological studies on proteinases produced by Aeromonas salmonicida and other aeromonads.
作者信息
Sandvik O, Hagen O
出版信息
Acta Vet Scand. 1968;9(1):1-9. doi: 10.1186/BF03547885.
A total of 12 strains of the genus Aeromonas were all good producers of so-called caseinate precipitating enzyme (CP-enzymes). CP-enzymes produced by different Aeromonas species have been serologically compared. The technique is a special immuno-electro-phoretic procedure, in which the proteolytic activity is neutralized by specific antiserums. No intergeneric cross-reactions could be demonstrated between enzymes produced by members of the genera Aeromonas, Pseudomonas, Proteus and Aerobacter. Interspecies cross-reactions occurred between enzymes of Aeromonas salmonicida and the species Ae. liquefaciens, Aa. punctata and Ae. hydrophila, whereas CP-enzymes of Ae. proteolytica were not found to be serologically related to those of the other species of the genus. However, the liquefaciens–punctata–hydrophila group could be enzymo-serologically differentiated from Ae. salmonicida, because that group in addition to the common enzyme fraction also possesses a fraction not found in Ae. salmonicida. The enzymo-serological procedure has been found a convenient tool in the identification of Ae. salmonicida and other aeromonads.
总共12株气单胞菌属菌株都是所谓酪蛋白沉淀酶（CP酶）的良好产生菌。已对不同气单胞菌属物种产生的CP酶进行了血清学比较。该技术是一种特殊的免疫电泳程序，其中蛋白水解活性被特异性抗血清中和。在气单胞菌属、假单胞菌属、变形杆菌属和埃希氏杆菌属成员产生的酶之间未发现属间交叉反应。杀鲑气单胞菌的酶与嗜水气单胞菌、点状气单胞菌和液化气单胞菌的酶之间发生种间交叉反应，而解蛋白气单胞菌的CP酶在血清学上与该属其他物种的酶无关。然而，液化气单胞菌–点状气单胞菌–嗜水气单胞菌组在酶血清学上可与杀鲑气单胞菌区分开来，因为该组除了有共同的酶组分外，还拥有杀鲑气单胞菌中未发现的一个组分。酶血清学程序已被证明是鉴定杀鲑气单胞菌和其他气单胞菌的一种便捷工具。
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