洗涤剂亲水亲油平衡值与枯草芽孢杆菌膜中D-丙氨酸羧肽酶增溶及稳定化的关系。
Relation of detergent HLB number to solubilization and stabilization of D-alanine carboxypeptidase from Bacillus subtilis membranes.
作者信息
Umbreit J N, Strominger J L
出版信息
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2997-3001. doi: 10.1073/pnas.70.10.2997.
Abstract
The ability of various nonionic detergents to solubilize D-alanine carboxypeptidase and other membrane-bound enzymes was correlated to a physical property of the detergent, the HLB number. Only a fairly narrow range of detergents were effective solubilizing agents. Purified carboxypeptidase required either a detergent or detergent plus a lipid fraction for stability. Only those detergents effective in solubilizing the enzyme were effective in stabilizing it.
摘要
各种非离子型去污剂溶解D-丙氨酸羧肽酶及其他膜结合酶的能力与去污剂的一种物理性质——亲水亲油平衡值（HLB值）相关。只有相当窄范围的去污剂是有效的增溶剂。纯化的羧肽酶需要一种去污剂或去污剂加一种脂质组分来保持稳定。只有那些能有效溶解该酶的去污剂才能有效使其稳定。
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