藤黄微球菌中去污剂增溶的磷酸乙酰胞壁酰五肽转位酶的复杂脂质需求
Complex lipid requirements for detergent-solubilized phosphoacetylmuramyl-pentapeptide translocase from Micrococcus luteus.
作者信息
Umbreit J N, Strominger J L
出版信息
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1972-4. doi: 10.1073/pnas.69.7.1972.
Phospho-MurNAc-pentapeptide translocase activity in the membrane of M. luteus was lost upon addition of the detergent, Triton X-100, but could be restored by addition of lipid fractions to the assay. By assay in the presence of lipid, the activity of the Triton-solubilized enzyme could be measured. The synthesis of C(55)-isoprenyl-P-P-MurNAc-pentapeptide from UDP-MurNAc-pentapeptide required C(55)-isoprenyl-P, and was stimulated by a neutral lipid. The exchange reaction of UDP-MurNAc-pentapeptide with UMP required a polar lipid fraction, but the reaction was not affected by C(55)-isoprenyl-P or the neutral lipid. Thus, measurement of activity of the detergent-solubilized enzyme requires addition of three lipids, the lipid substrate (C(55)-isoprenyl-P), the neutral lipid, and a polar lipid.
在藤黄微球菌膜中的磷酸化胞壁酰五肽转位酶活性在加入去污剂Triton X-100后丧失，但可通过向测定体系中添加脂质组分来恢复。通过在有脂质存在的情况下进行测定，可以测量Triton增溶酶的活性。由UDP-胞壁酰五肽合成C(55)-异戊二烯基-P-P-胞壁酰五肽需要C(55)-异戊二烯基-P，并且受到一种中性脂质的刺激。UDP-胞壁酰五肽与UMP的交换反应需要一种极性脂质组分，但该反应不受C(55)-异戊二烯基-P或中性脂质的影响。因此，测定去污剂增溶酶的活性需要添加三种脂质，即脂质底物（C(55)-异戊二烯基-P）、中性脂质和极性脂质。
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