Sargent M G
J Bacteriol. 1973 Nov;116(2):736-40. doi: 10.1128/jb.116.2.736-740.1973.
A simple method of potentially wide applicability for obtaining synchronous cultures of Bacillus subtilis based on size selection is described. Using glass fiber filters, a population (about 1 to 2% of the parent population) can be obtained substantially enriched for small cells which grow synchronously. A method for rapidly concentrating dilute suspensions of cells is described.
描述了一种基于大小选择获得枯草芽孢杆菌同步培养物的具有潜在广泛适用性的简单方法。使用玻璃纤维滤器,可以获得大量富集的小细胞群体(约为亲代群体的1%至2%),这些小细胞能同步生长。还描述了一种快速浓缩细胞稀释悬浮液的方法。