Chan E W, Dunkel V C
Appl Microbiol. 1973 Sep;26(3):386-90. doi: 10.1128/am.26.3.386-390.1973.
A microtissue culture method for the plaque assay of Herpesvirus saimiri has been developed. Virus titrations carried out in Microtest II tissue culture plates (Falcon) yielded reproducible results that agreed well with those obtained by employing macrocultures. The described method is quantitative, reproducible, economical, and suitable for routine assay of large numbers of virus samples.
已开发出一种用于赛米利疱疹病毒蚀斑测定的微组织培养方法。在Microtest II组织培养板(Falcon)中进行的病毒滴定产生了可重复的结果,这些结果与采用大培养物获得的结果非常吻合。所描述的方法是定量的、可重复的、经济的,并且适用于大量病毒样本的常规测定。