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人类和猿猴巨细胞病毒的微量培养蚀斑试验

Microculture plaque assay for human and simian cytomegaloviruses.

作者信息

Chiba S, Striker R L, Benyesh-Melnick M

出版信息

Appl Microbiol. 1972 Apr;23(4):780-3. doi: 10.1128/am.23.4.780-783.1972.

DOI:10.1128/am.23.4.780-783.1972
PMID:4336229
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC380435/
Abstract

The plaque assay for human and simian cytomegaloviruses routinely carried out in 60-mm petri dishes (macrocultures) has been adapted for use in microcultures in flat-bottom 16-mm circular wells of disposable plastic trays. Virus titrations and serum neutralization assays carried out in microcultures yielded reproducible results that were identical to those obtained in macrocultures.

摘要

用于人类和猿猴巨细胞病毒的蚀斑测定法通常在60毫米培养皿(大培养物)中进行,现已适用于一次性塑料托盘平底16毫米圆形孔中的微培养物。在微培养物中进行的病毒滴定和血清中和试验产生了可重复的结果,这些结果与在大培养物中获得的结果相同。

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本文引用的文献

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VIRUSES IN CELL CULTURES OF KIDNEYS OF CHILDREN WITH CONGENITAL HEART MALFORMATIONS AND OTHER DISEASES.先天性心脏畸形及其他疾病患儿肾脏细胞培养中的病毒
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A PLAQUE REDUCTION NEUTRALIZATION TEST FOR HUMAN CYTOMEGALOVIRUS.人巨细胞病毒蚀斑减少中和试验
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Cytopathogenic agent resembling human salivary gland virus recovered from tissue cultures of human adenoids.从人腺样体组织培养物中分离出的类似人类涎腺病毒的细胞致病因子。
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The measurement of specific antibodies in Bolivian hemorrhagic fever by neutralization of virus plaques.通过病毒蚀斑中和法检测玻利维亚出血热中的特异性抗体。
Proc Soc Exp Biol Med. 1969 Mar;130(3):1013-9. doi: 10.3181/00379727-130-33711.
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Complement-requiring neutralizing antibodies in hyperimmune sera to human cytomegaloviruses.人巨细胞病毒高免疫血清中需要补体的中和抗体。
J Immunol. 1971 Dec;107(6):1618-30.
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Miniculture plaquing technique for mouse cytomegalovirus.小鼠巨细胞病毒的微量培养噬斑技术
Appl Microbiol. 1971 Jul;22(1):139-40. doi: 10.1128/am.22.1.139-140.1971.
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Plaque assay of cytomegalovirus strains of human origin.人源巨细胞病毒毒株的空斑试验
Proc Soc Exp Biol Med. 1970 Nov;135(2):253-8. doi: 10.3181/00379727-135-35031.
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J Immunol. 1967 Dec;99(6):1106-14.
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Interactions of human cytomegalovirus with human fibroblasts.人巨细胞病毒与人成纤维细胞的相互作用。
J Bacteriol. 1966 Jan;91(1):213-20. doi: 10.1128/jb.91.1.213-220.1966.