Pennington T H, Follett E A
J Virol. 1974 Feb;13(2):488-93. doi: 10.1128/JVI.13.2.488-493.1974.
The growth of vaccinia virus in monolayers of BSC-1 cells enucleated by centrifugation in the presence of cytochalasin B has been studied. No evidence for the production of infectious virus in these cells was obtained, and the production of virus particles was reduced to 8.3% compared with the yield from cytochalasin-treated, uncentrifuged cells. Virus DNA and early and late polypeptides were synthesized with normal timing in enucleate cells, but in reduced amounts; cleavage of structural polypeptide precursors P4a and Px also occurred in enucleate cells. Factories containing immature virus particles were demonstrated in enucleate cells by electron microscopy; these factories were reduced in number and size compared with those found in cytochalasin-treated, uncentrifuged cells.
研究了在细胞松弛素B存在下通过离心去核的BSC - 1细胞单层中痘苗病毒的生长情况。未获得这些细胞产生传染性病毒的证据，与经细胞松弛素处理但未离心的细胞相比，病毒颗粒的产量降至8.3%。在去核细胞中，病毒DNA以及早期和晚期多肽按正常时间合成，但量减少；结构多肽前体P4a和Px的切割也发生在去核细胞中。通过电子显微镜在去核细胞中证实了含有未成熟病毒颗粒的工厂；与在经细胞松弛素处理但未离心的细胞中发现的工厂相比，这些工厂的数量和大小减少。
