Shakhova T V, Rassulin Iu A, Konovalov S A
Prikl Biokhim Mikrobiol. 1979 Sep-Oct;15(5):702-6.
From the culture liquid filtrate of Aspergillus awamori 78-2 a preparation of acid proteinase was isolated and its properties were described. This producer was shown to have a peculiar capacity of synthesizing under submerged conditions extracellular acid proteinase as the sole proteolytic enzyme. The preparation of acid proteinase showed a comparatively high hydrolyzing capacity. Under optimal conditions the enzyme degraded hemoglobin and albumin almost completely. The possibility of purifying and concentrating acid proteinase on the ion-exchange resin KB 51 X 2 was explored.
从泡盛曲霉78-2的培养液滤液中分离出一种酸性蛋白酶制剂，并对其性质进行了描述。该菌株在深层培养条件下具有独特的合成细胞外酸性蛋白酶作为唯一蛋白水解酶的能力。酸性蛋白酶制剂表现出较高的水解能力。在最佳条件下，该酶几乎能完全降解血红蛋白和白蛋白。研究了在离子交换树脂KB 51X 2上纯化和浓缩酸性蛋白酶的可能性。
