一种用于确定混合性、非竞争性和反竞争性抑制剂抑制常数的简单图解法。
A simple graphical method for determining the inhibition constants of mixed, uncompetitive and non-competitive inhibitors.
作者信息
Cornish-Bowden A
出版信息
Biochem J. 1974 Jan;137(1):143-4. doi: 10.1042/bj1370143.
Abstract
A new method is described for plotting kinetic results for inhibited enzyme-catalysed reactions. It provides a simple way of determining the inhibition constant, K'(i), of an uncompetitive, mixed or non-competitive inhibitor.
摘要
描述了一种绘制酶催化抑制反应动力学结果的新方法。它提供了一种确定非竞争性、混合型或非竞争性抑制剂的抑制常数K'(i)的简单方法。
相似文献
1
A simple graphical method for determining the inhibition constants of mixed, uncompetitive and non-competitive inhibitors.一种用于确定混合性、非竞争性和反竞争性抑制剂抑制常数的简单图解法。
Biochem J. 1974 Jan;137(1):143-4. doi: 10.1042/bj1370143.
2
A graphical method for determining inhibition constants.一种用于确定抑制常数的图形方法。
J Enzyme Inhib Med Chem. 2009 Dec;24(6):1288-90. doi: 10.3109/14756360902829766.
3
The specific velocity plot. A graphical method for determining inhibition parameters for both linear and hyperbolic enzyme inhibitors.特定速度图。一种用于确定线性和双曲线型酶抑制剂抑制参数的图形方法。
Eur J Biochem. 1981 Sep;119(1):9-14. doi: 10.1111/j.1432-1033.1981.tb05570.x.
4
Kinetic study of an enzyme-catalysed reaction in the presence of novel irreversible-type inhibitors that react with the product of enzymatic catalysis.在存在与酶催化产物发生反应的新型不可逆型抑制剂的情况下，对酶催化反应进行动力学研究。
Bull Math Biol. 1995 Jan;57(1):157-68. doi: 10.1007/BF02458321.
5
A new plot for multiple enzyme inhibition.一种多重酶抑制的新策略。
Biochem Int. 1983 Aug;7(2):247-53.
6
Progress-curve equations for reversible enzyme-catalysed reactions inhibited by tight-binding inhibitors.被紧密结合抑制剂抑制的可逆酶催化反应的进程曲线方程。
Biochem J. 1990 Feb 1;265(3):647-53. doi: 10.1042/bj2650647.
7
A graphical method for determining inhibition parameters for partial and complete inhibitors.一种用于确定部分抑制剂和完全抑制剂抑制参数的图解方法。
Biochem J. 1987 Dec 15;248(3):815-20. doi: 10.1042/bj2480815.
8
Combination plots as graphical tools in the study of enzyme inhibition.组合图作为酶抑制研究中的图形工具。
Biochem J. 1995 Nov 1;311 ( Pt 3)(Pt 3):981-5. doi: 10.1042/bj3110981.
9
A new plot for the kinetic analysis of dead-end enzyme inhibitors.一种用于终产物酶抑制剂动力学分析的新方法。
Biochem Int. 1985 Jul;11(1):11-9.
10
Inhibition of enzymes by metal ion-chelating reagents. Theory and new graphical methods of study.金属离子螯合试剂对酶的抑制作用。研究的理论与新的图解方法。
Biochem J. 1974 Jan;137(1):55-60. doi: 10.1042/bj1370055.
引用本文的文献
1
The mycotoxin Beauvericin is an uncompetitive inhibitor of Cathepsin B.霉菌毒素白僵菌素是组织蛋白酶B的非竞争性抑制剂。
Protein Sci. 2025 Jun;34(6):e70173. doi: 10.1002/pro.70173.
2
Design and Synthesis of Novel 6-(Substituted phenyl)-[1,3]dioxolo[4',5':4,5]benzo[1,2-]thiazole Compounds as Tyrosinase Inhibitors: In Vitro and In Vivo Insights.新型6-(取代苯基)-[1,3]二氧杂环戊并[4',5':4,5]苯并[1,2-]噻唑化合物作为酪氨酸酶抑制剂的设计与合成：体外和体内研究洞察
Molecules. 2025 Mar 30;30(7):1535. doi: 10.3390/molecules30071535.
3
Caffeic Acid Phenethyl Ester (CAPE) Inhibits Arginase Activity and Growth of Promastigotes and Intracellular Amastigotes.咖啡酸苯乙酯（CAPE）抑制精氨酸酶活性以及前鞭毛体和细胞内无鞭毛体的生长。
Pathogens. 2025 Apr 15;14(4):384. doi: 10.3390/pathogens14040384.
4
Ionic and Non-Ionic Counterparts Based on Bis(Uracilyl)Alkane Moiety with Highest Selectivity Towards Acetylcholinesterase for Protection Against Organophosphate Poisoning and Treating Alzheimer's Disease.基于双（尿嘧啶基）烷烃部分的离子型和非离子型对应物，对乙酰胆碱酯酶具有最高选择性，用于预防有机磷中毒和治疗阿尔茨海默病。
Int J Mol Sci. 2025 Apr 16;26(8):3759. doi: 10.3390/ijms26083759.
5
Intramolecular epistasis correlates with divergence of specificity in promiscuous and bifunctional NSAR/OSBS enzymes.分子内上位性与混杂和双功能NSAR/OSBS酶特异性的差异相关。
Protein Sci. 2025 May;34(5):e70113. doi: 10.1002/pro.70113.
6
Melatonin rescues cell respiration impaired by hypoxia/reoxygenation in aortic endothelial cells and affects the mitochondrial bioenergetics targeting the FF-ATPase.褪黑素可挽救主动脉内皮细胞中因缺氧/复氧而受损的细胞呼吸，并影响靶向F型ATP酶的线粒体生物能量学。
Redox Biol. 2025 May;82:103605. doi: 10.1016/j.redox.2025.103605. Epub 2025 Mar 20.
7
Conjugation of Triterpenic Acids with 3-Aminoquinuclidine Moiety: An Approach to Acetylcholinesterase Mixed or Uncompetitive Type Inhibitors.三萜酸与3-氨基奎宁环部分的缀合：一种制备乙酰胆碱酯酶混合型或非竞争性抑制剂的方法。
Molecules. 2024 Dec 29;30(1):95. doi: 10.3390/molecules30010095.
8
Polystyrene-Poly(acrylic acid) Block Copolymers for Encapsulation of Butyrylcholinesterase into Injectable Nanoreactors.用于将丁酰胆碱酯酶封装到可注射纳米反应器中的聚苯乙烯-聚丙烯酸嵌段共聚物。
Biomolecules. 2024 Dec 5;14(12):1555. doi: 10.3390/biom14121555.
9
Assessing the Potential of 1,2,3-Triazole-Dihydropyrimidinone Hybrids Against Cholinesterases: In Silico, In Vitro, and In Vivo Studies.评估 1,2,3-三唑并[4,5-d]嘧啶二酮杂合体对胆碱酯酶的潜在抑制作用：计算机模拟、体外和体内研究。
Int J Mol Sci. 2024 Oct 17;25(20):11153. doi: 10.3390/ijms252011153.
10
Enzymolysis Modes Trigger Diversity in Inhibitor-α-Amylase Aggregating Behaviors and Activity Inhibition: A New Insight Into Enzyme Inhibition.酶解模式触发抑制剂-α-淀粉酶聚集行为和活性抑制的多样性：对酶抑制作用的新认识。
Adv Sci (Weinh). 2024 Nov;11(41):e2404127. doi: 10.1002/advs.202404127. Epub 2024 Sep 5.
本文引用的文献
1
Statistical analysis of enzymic steady-state rate data.酶促稳态速率数据的统计分析。
C R Trav Lab Carlsberg. 1961;32:185-214.
2
A COMPARISON OF ESTIMATES OF MICHAELIS-MENTEN KINETIC CONSTANTS FROM VARIOUS LINEAR TRANSFORMATIONS.基于各种线性变换的米氏动力学常数估计值比较
J Biol Chem. 1965 Feb;240:863-9.
3
Statistical estimations in enzyme kinetics.酶动力学中的统计估计
Biochem J. 1961 Aug;80(2):324-32. doi: 10.1042/bj0800324.
4
The determination of enzyme inhibitor constants.酶抑制剂常数的测定
Biochem J. 1953 Aug;55(1):170-1. doi: 10.1042/bj0550170.
5
Limitations of he Dixon plot for ascertaining naure of enzyme inhibition.狄克逊图在确定酶抑制性质方面的局限性。
Tex Rep Biol Med. 1969 Summer;27(2):483-8.
Zotero 插件