Jennings P B, Crumrine M H, Fischer G W, Cunningham T C
Appl Microbiol. 1974 Feb;27(2):297-9. doi: 10.1128/am.27.2.297-299.1974.
A blood culture technique that utilized small arterial blood samples or peripheral capillary blood was tested in beagle dogs and pig-tailed macaque monkeys. A bolus of 2.0 x 10(7)Escherichia coli (ATCC 25922) was injected intravenously into five animals of each species. Blood samples were taken before injection of the organisms and 10, 15, 20, 30, 60, and 120 min after injection. Arterial blood samples (2.0 and 0.2 ml) and peripheral capillary samples (0.14 ml) were taken at each sampling time. Pour plates were prepared from arterial blood for colony counts. All three blood sampling methods were equally effective in detecting sepsis when 10 or more organisms per ml of blood were present. Below this level, the 2.0-ml sample was more effective. Contamination of the peripheral sample with air or skin contaminants was a problem.
一种利用小动脉血样本或外周毛细血管血的血培养技术在比格犬和食蟹猕猴身上进行了测试。将2.0×10⁷大肠杆菌（ATCC 25922）静脉注射到每个物种的五只动物体内。在注射细菌前以及注射后10、15、20、30、60和120分钟采集血样。在每个采样时间采集动脉血样本（2.0和0.2毫升）和外周毛细血管样本（0.14毫升）。从动脉血制备倾注平板进行菌落计数。当每毫升血液中存在10个或更多细菌时，所有三种血样采集方法在检测败血症方面同样有效。低于此水平时，2.0毫升样本更有效。外周样本被空气或皮肤污染物污染是一个问题。
