Müller W E, Zahn R K, Arendes J, Kurelec B, Steffen R, Müller I
Biochim Biophys Acta. 1979 Mar 8;551(2):363-7. doi: 10.1016/0005-2736(89)90012-6.
In the extracellular space of the tissue of the sponge Geodia cydonium, circular proteid particles are found which carry as subunits the aggregation factor and a series of glycosyltransferases. Using the technique of velocity sucrose gradient centrifugation, the sedimentation coefficient (S020,w) of the particle-monosomes was determined to be 90. By means of the Svedberg equation a molecular weight of 1.3 . 10(8) daltons could be estimated. The monosomes aggregate in the presence of Ca2+ to higher complexes via disomes, trisomes, and pentasomes. The complexes can be redissociated by dodecyl sulfate but not by EDTA. During the Ca2+-mediated self-aggregation, the particles lose their biological activity with respect to their aggregation promoting function.
在海绵Geodia cydonium组织的细胞外空间中，发现了圆形蛋白质颗粒，这些颗粒作为亚基携带聚集因子和一系列糖基转移酶。使用速度蔗糖梯度离心技术，确定颗粒单体的沉降系数（S020，w）为90。通过斯维德伯格方程，可以估计分子量为1.3×10⁸道尔顿。单体在Ca²⁺存在下通过二聚体、三聚体和五聚体聚合成更高的复合物。这些复合物可以被十二烷基硫酸盐重新解离，但不能被EDTA解离。在Ca²⁺介导的自聚集过程中，颗粒丧失了其促进聚集功能的生物活性。
