Müller W E, Zahn R K, Kurelec B, Müller I
Physiologisch-Chemisches Institut, Johannes-Gutenberg-Universität, Mainz, Germany.
Exp Cell Res. 1978 May;113(2):409-14. doi: 10.1016/0014-4827(78)90382-8.
Single cells from the siliceous sponge Geodia cydonium, obtained after chemical dissociation, reaggregate in the presence of the aggregation factor in Ca2+-containing medium to large aggregates. It was found that polyvalent organic cations (polylysine, spermine, spermidine, putrescine) enhance the Ca2+-mediated cell aggregation. In Ca2+-free medium these compounds also cause reaggregation; aggregates of a diameter up to 800 micron are formed within 120 min. Proteins, containing basic groups of amino acid residues have no influence on cell aggregation. Monovalent cations inhibit the reaggregation process. The enhancing effect of polyvalent organic cations on cell aggregation is dependent on the presence of the soluble aggregation factor. From the findings that polycations do not alter the duration of the lag phase (a characteristic of the aggregation factor-mediated Geodia cell reaggregation) and act in cooperation with the aggregation receptor, we assume that the polycations bind between the aggregation factor and the aggregation receptor.
经化学解离后获得的硅质海绵地穴海绵（Geodia cydonium）的单细胞，在含Ca2+的培养基中，于聚集因子存在的情况下重新聚集形成大聚集体。研究发现，多价有机阳离子（聚赖氨酸、精胺、亚精胺、腐胺）可增强Ca2+介导的细胞聚集。在无Ca2+的培养基中，这些化合物也会引起重新聚集；在120分钟内可形成直径达800微米的聚集体。含有氨基酸残基碱性基团的蛋白质对细胞聚集没有影响。单价阳离子会抑制重新聚集过程。多价有机阳离子对细胞聚集的增强作用取决于可溶性聚集因子的存在。从多阳离子不会改变延迟期的持续时间（这是聚集因子介导的地穴海绵细胞重新聚集的一个特征）且与聚集受体协同作用的发现，我们推测多阳离子结合在聚集因子和聚集受体之间。
