Eisenberg R C, Butters S J, Quay S C, Friedman S B
J Bacteriol. 1974 Oct;120(1):147-53. doi: 10.1128/jb.120.1.147-153.1974.
Pseudomonas fluorescens ATCC 13525 and a particulate glucose oxidase (d-glucose:oxygen oxidoreductase, EC 1.1.3.4) mutant of this organism, gox-7, were examined to determine if glucose oxidation via particulate glucose oxidase is a required first step for glucose uptake. Initial [(14)C]glucose-uptake rates in parent and gox-7 cells were qualitatively similar. Initial [(14)C]glucose-uptake product analysis revealed that glucose was accumulated via active transport and was rapidly metabolized to glucose-6-phosphate and gluconate-6-phosphate in both parent and gox-7 cells. Cell extracts contained soluble adenosine 5'-triphosphate specific kinase activity for phosphorylation of glucose. Glucose uptake was induced by glucose and not gluconate, thus, establishing independent regulation of glucose transport and glucose catabolism in p. fluorescens. The results prove that glucose oxidase was not an obligatory reaction for glucose carbon permeation in P. fluorescens. A general unifying scheme for glucose utilization in the aerobic fluorescent pseudomonads is suggested for the purpose of clarifying glucose uptake in these bacteria.
对荧光假单胞菌ATCC 13525及其该菌株的一种颗粒状葡萄糖氧化酶（D-葡萄糖：氧氧化还原酶，EC 1.1.3.4）突变体gox-7进行了检测，以确定通过颗粒状葡萄糖氧化酶进行的葡萄糖氧化是否是葡萄糖摄取的必需第一步。亲本细胞和gox-7细胞的初始[¹⁴C]葡萄糖摄取率在定性上相似。初始[¹⁴C]葡萄糖摄取产物分析表明，葡萄糖通过主动转运积累，并在亲本细胞和gox-7细胞中迅速代谢为6-磷酸葡萄糖和6-磷酸葡萄糖酸。细胞提取物含有用于葡萄糖磷酸化的可溶性腺苷5'-三磷酸特异性激酶活性。葡萄糖摄取由葡萄糖而非葡萄糖酸盐诱导，因此，在荧光假单胞菌中建立了葡萄糖转运和葡萄糖分解代谢的独立调节。结果证明，葡萄糖氧化酶对于荧光假单胞菌中葡萄糖碳的渗透不是必需反应。为了阐明这些细菌中的葡萄糖摄取，提出了需氧荧光假单胞菌中葡萄糖利用的一般统一方案。
