氧化醋杆菌的肌醇脱氢酶。膜结合酶增溶条件的优化。
Myo-Inositol dehydrogenase(s) from Acetomonas oxydans. Optimization of conditions for solubilization of membrane-bound enzyme.
作者信息
Criddle W J, Fry J C, Keaney M M
出版信息
Biochem J. 1974 Mar;137(3):449-52. doi: 10.1042/bj1370449.
Abstract
Methods are described for the first reported successful isolation of the soluble form of the membrane-bound myo-inositol dehydrogenase(s) from Acetomonas oxydans. Conditions for optimum yields of active enzyme in a crude membrane-free protein extract were established. The relevant conditions are (a) cell rupture by solid-shearing, (b) solubilization of the complete 60000g pellet with sodium deoxycholate at pH7.2, (c) rapid separation of the released protein from sodium deoxycholate by gel chromatography.
摘要
本文描述了从氧化醋杆菌中首次成功分离出膜结合型肌醇脱氢酶可溶性形式的方法。确定了在无细胞粗蛋白提取物中获得活性酶最佳产量的条件。相关条件如下：(a) 通过固体剪切使细胞破裂；(b) 用脱氧胆酸钠在pH7.2条件下溶解完整的60000g沉淀；(c) 通过凝胶色谱法快速从脱氧胆酸钠中分离出释放的蛋白质。
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引用本文的文献
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Myo-inositol dehydrogenase(s) from Acetomonas oxydans.氧化醋杆菌中的肌醇脱氢酶
Mol Cell Biochem. 1977 May 31;16(1):3-8. doi: 10.1007/BF01769832.
本文引用的文献
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Bacterial enzyme preparations oxidizing inositol and their inhibition by colchicine.氧化肌醇的细菌酶制剂及其被秋水仙碱的抑制作用。
Nature. 1951 Dec 1;168(4283):955-7. doi: 10.1038/168955b0.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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The survival of bacteria during and after drying.细菌在干燥过程中及干燥后的存活情况。
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Sub-cellular particles and the nicotinic acid hydroxylase system in extracts of.提取物中的亚细胞颗粒与烟酸羟化酶系统。
Biochim Biophys Acta. 1959 Aug;34:354-72. doi: 10.1016/0006-3002(59)90288-4.
5
[On the cyclic alcohol dehydrogenases of Acetobacter suboxydans I].
Helv Chim Acta. 1967 Sep;50(7):1801-10. doi: 10.1002/hlca.19670500713.
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