Criddle W J, Fry J C, Keaney M M
Biochem J. 1974 Mar;137(3):449-52. doi: 10.1042/bj1370449.
Methods are described for the first reported successful isolation of the soluble form of the membrane-bound myo-inositol dehydrogenase(s) from Acetomonas oxydans. Conditions for optimum yields of active enzyme in a crude membrane-free protein extract were established. The relevant conditions are (a) cell rupture by solid-shearing, (b) solubilization of the complete 60000g pellet with sodium deoxycholate at pH7.2, (c) rapid separation of the released protein from sodium deoxycholate by gel chromatography.
本文描述了从氧化醋杆菌中首次成功分离出膜结合型肌醇脱氢酶可溶性形式的方法。确定了在无细胞粗蛋白提取物中获得活性酶最佳产量的条件。相关条件如下：(a) 通过固体剪切使细胞破裂；(b) 用脱氧胆酸钠在pH7.2条件下溶解完整的60000g沉淀；(c) 通过凝胶色谱法快速从脱氧胆酸钠中分离出释放的蛋白质。
